Cloned (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
- |
Pseudomonas mendocina |
Crystallization (Comment) | Organism |
---|---|
homology modeling. Residue I100 aids in steering the substrate into the active site at the end of the long entrance channel | Pseudomonas mendocina |
Protein Variants | Comment | Organism |
---|---|---|
I100A | mutant shows similar rates as wild-type | Pseudomonas mendocina |
I100D | mutation improves both reaction rate and enantioselectivity | Pseudomonas mendocina |
I100G | mutation increases the wild-type oxidation rate of methyl phenyl sulfide by 1.7fold, and the enantiomeric excess rises from 86% to 98% pro-S. I100G oxidizes methyl para-tolyl sulfide 11 times faster than the wild type does and changes the selectivity from 41% pro-R to 77% pro-S | Pseudomonas mendocina |
I100S | mutation improves both reaction rate and enantioselectivity | Pseudomonas mendocina |
I100V | mutation improves both reaction rate and enantioselectivity | Pseudomonas mendocina |
V106A | initial thioanisole sulfoxidation is improved by 1.65fold | Burkholderia cepacia |
V106E | initial thioanisole sulfoxidation is improved by 1.72fold | Burkholderia cepacia |
V106L | initial thioanisole sulfoxidation is decreased by 0.43fold | Burkholderia cepacia |
V106M | mutant oxidizes methyl phenyl sulfide to the corresponding sulfoxide at a rate of 3.0 nmol/min/mg protein compared with 1.6 for the wild-type enzyme, and the enantiomeric excess (pro-S) increases from 51% for the wild type to 88% for this mutant. Function of residue V106 is the proper positioning or docking of the substrate with respect to the diiron atoms | Burkholderia cepacia |
V106S | initial thioanisole sulfoxidation is decreased by 0.8fold | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
methyl 4-tolyl sulfide | whole-cell assay, 25°C, pH not specified in the publication | Pseudomonas mendocina | |
0.068 | - |
methyl 4-tolyl sulfide | whole-cell assay, 25°C, pH not specified in the publication | Burkholderia cepacia | |
0.115 | - |
methyl phenyl sulfide | whole-cell assay, 25°C, pH not specified in the publication | Pseudomonas mendocina | |
0.129 | - |
methyl phenyl sulfide | whole-cell assay, 25°C, pH not specified in the publication | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q9ANX2 | isoform TomA3 | - |
Burkholderia cepacia G4 | Q9ANX2 | isoform TomA3 | - |
Pseudomonas mendocina | Q00456 | isoform TmoA | - |
Pseudomonas mendocina KR1 | Q00456 | isoform TmoA | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
substrate methyl 4-tolyl sulfide, whole-cell assay, 25°C, pH not specified in the publication | Burkholderia cepacia |
0.5 | - |
substrate methyl 4-tolyl sulfide, whole-cell assay, 25°C, pH not specified in the publication | Pseudomonas mendocina |
1.6 | - |
substrate methyl phenyl sulfide, whole-cell assay, 25°C, pH not specified in the publication | Burkholderia cepacia |
1.6 | - |
substrate methyl phenyl sulfide, whole-cell assay, 25°C, pH not specified in the publication | Pseudomonas mendocina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl 4-tolyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina | methyl 4-tolyl sulfoxide + NAD+ + H2O | (pro-R)-sulfoxide, 41% enantiomeric excess | ? | |
methyl 4-tolyl sulfide + NADH + H+ + O2 | - |
Burkholderia cepacia | methyl 4-tolyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 11% enantiomeric excess | ? | |
methyl 4-tolyl sulfide + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | methyl 4-tolyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 11% enantiomeric excess | ? | |
methyl 4-tolyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | methyl 4-tolyl sulfoxide + NAD+ + H2O | (pro-R)-sulfoxide, 41% enantiomeric excess | ? | |
methyl phenyl sulfide + NADH + H+ + O2 | - |
Burkholderia cepacia | methyl phenyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 51% enantiomeric excess | ? | |
methyl phenyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina | methyl phenyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 86% enantiomeric excess | ? | |
methyl phenyl sulfide + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | methyl phenyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 51% enantiomeric excess | ? | |
methyl phenyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | methyl phenyl sulfoxide + NAD+ + H2O | (pro-S)-sulfoxide, 86% enantiomeric excess | ? | |
toluene + NADH + H+ + O2 | - |
Burkholderia cepacia | 4-methylphenol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methylphenol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | 4-methylphenol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-methylphenol + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TmoA | - |
Pseudomonas mendocina |
TomA3 | - |
Burkholderia cepacia |